Josephine F. Trott, Ph.D.

Education

BScAgr (Hons, Convocation Medal, University Medal) (Agricultural Genetics major), University of Sydney, Australia

PhD, Zoology and Molecular Biology, University of Melbourne, Australia

Research

Evaluation of the offspring of a genome edited polled bull

Publications

Carmen M. Banks*, Josephine F. Trott*, and Russell C. Hovey. 2024. The Prolactin Receptor: A cross-species comparison of gene structure, transcriptional regulation, tissue-specificity, and genetic variation. Journal of Neuroendocrinology.;e13385. https://doi.org/10.1111/jne.13385

Anna S. Sadovnikova, Sergio Garcia, Josephine F. Trott, Alice T. Mathews, Monica Britton, Blythe P. Durbin-Johnson, and Russell C. Hovey. 2022. Transcriptomic changes underlying glucocorticoid-induced suppression of milk production by dairy cows. Frontiers in Genetics. 13: 1072853. https://doi.org/10.3389/fgene.2022.1072853

Hennig, S.L., McNabb, B.R., Trott, J.F., Van Eenennaam, A.L. and Murray, J.D. 2022. LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the Celtic polled allele. Scientific Reports, 12: 7627. https://doi.org/10.1038/s41598-022-11669-9

Trott, J.F., Young, A.E., McNabb, B.R., Yang, X., Bishop, T.F. and Van Eenennaam, A.L. 2022. Animal health and food safety analyses of six offspring of a genome-edited hornless bull. GEN Biotechnology, 1(2): 192-206. http://doi.org/10.1089/genbio.2022.0008

Alison L. Van Eenennaam, Sadie L. Hennig, Josephine F. Trott, Bret R. McNabb, Joseph R. Owen, Amy E. Young, Maci L. Mueller, Jason C. Lin, James D. Murray. 2022. Functional Analysis and Characterization of the Hornless Phenotype in Cattle. Plant and Animal Genome XXIX Conference.

Josephine F. Trott, Anke Schennink, Katherine C. Horigan, Danielle G. Lemay, Julia R. Cohen, Thomas R. Famula, Julie A. Dragon and Russell C. Hovey. 2022. Unique transcriptomic changes underlie hormonal interactions during mammary histomorphogenesis in female pigs. Endocrinology, 163(3): 1-27. doi: 10.1210/endocr/bqab256 

Trott, J.F., Young, A.E., McNabb, B.R., Yang, X., Bishop, T.F. and Van Eenennaam, A.L. Comparative Evaluation Of Human-Edible Animal Products Derived From Offspring Of Genome Edited And Control Cattle. 2021. Journal of Animal Science, 99(Supplement_3): 11-12.

G.E. Berryhill, J.M. Gloviczki, J.F. Trott, J. Kraft, A.L. Lock, and R.C. Hovey. In utero exposure to trans-10, cis-12 conjugated linoleic acid modifies postnatal development of the mammary gland and its hormone responsiveness. 2021. Journal of Mammary Gland Biology and Neoplasia, Sep;26(3):263-276. doi: 10.1007/s10911-021-09499-y. Epub 2021 Oct 6. PMID: 34617201; PMCID: PMC8566432.

Katherine Bernardo, Russell Hovey, Josephine Trott, Erin Wagner, Rebekah Karns, Sarah Riddle, Amy Thompson, Laura Ward, and Laurie Nommsen-Rivers. Hormone-Sensitive Gene Signatures in the Mammary Epithelial Cells of Lactating Women With Persistent Low Milk Production. 2021 Current Developments in Nutrition, 5(Supplement_2): 720. https://doi.org/10.1093/cdn/nzab046_017

Frederick Schozer, Patrick Tso, Rebekah Karns, Erin Wagner, Rachel Walker, Russell Hovey, Josephine Trott, Sarah Riddle, Amy Thompson, Laura Ward and Laurie Nommsen-Rivers. Chronic Elevation of Tumor Necrosis Factor-Alpha Suppresses Lactation via Downregulation of Lipoprotein Lipase. 2021. Current Developments in Nutrition, 5(Supplement_2): 814. https://doi.org/10.1093/cdn/nzab046_111

David Zilberman, Alison L. Van Eenennaam, Felipe De Figueiredo Silva, and Josephine F. Trott. The Costs of Overregulating Animal and Plant Biotechnology: Lessons from COVID-19. 2021. ARE Update, 24(4): 1-4. https://s.giannini.ucop.edu/uploads/pub/2021/04/22/v24n4_1.pdf

Van Eenennaam AL, De Figueiredo Silva F, Trott JF, Zilberman D. Genetic Engineering of Livestock: The Opportunity Cost of Regulatory Delay. Annu Rev Anim Biosci. 2021 Feb 16;9:453-478. Epub 2020 Nov 13. doi: 10.1146/annurev-animal-061220-023052. PMID: 33186503.

Owen JR, Hennig SL, McNabb BR, Mansour TA, Smith JM, Lin JC, Young AE, Trott JF, Murray JD, Delany ME, Ross PJ, Van Eenennaam AL. One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes. BMC Genomics. 2021 Feb 12;22(1):118. doi: 10.1186/s12864-021-07418-3. PMID: 33581720; PMCID: PMC7881600

Zilberman D, Van Eenennaam AL, Figueiredo Silva FD, Trott JF. The Costs of Overregulating Animal and Plant Biotechnology: Lessons from COVID-19. ARE Update. 2021; 24(4):1-4. 

Mathews, A.T., Banks, C.M, Trott, J.F., Saniz, R.D., Farmer, C., Pendergast, I.I. and Hovey, R.C. 2021 Metoclopramide induces preparturient, low-level hyperprolactinemia to increase milk production in primiparous sows. Domestic Animal Endocrinology 2021 Jan;74:106517. Epub 2020 Jul 3. doi: 10.1016/j.domaniend.2020.106517. PMID: 32739764.

Grace N. Elbert, Christina Valentine, Bruce Aronow, Russell Hovey, Josephine Trott, Erin Wagner, Rebekah Karns, Sarah Riddle, Amy Thompson, Laura P. Ward and Laurie Nommsen-Rivers. Expression Of Inflammation Associated Genes In The Mammary Gland Of Lactating Women With Elevated Plasma Triglycerides. 2021. Pediatrics, 147(3 MeetingAbstract): 315-316.

Susan G. Miszewski, Josephine F. Trott, Grace E. Berryhill, Lyvin Tat, Ralph Green, Alexander D. Borowsky, Joshua W. Miller, and Russell C. Hovey. Folate deficiency inhibits development of the mammary gland and its associated lymphatics in FVB mice. Journal of Nutrition, 2020 Aug 1;150(8):2120-2130. https://doi.org/10.1093/jn/nxaa154 PMID: 32510141.

Alice T Mathews, Carmen M Banks, Josephine F Trott, Roberto D Sainz, Chantal Farmer, Izabella I Pendergast, Russell C Hovey. Metoclopramide induces low-level hyperprolactinemia to increase milk production in sows. Journal of Animal Science, 98(Supplement_4): 179–180. https://doi.org/10.1093/jas/skaa278.330

Josephine F. Trott, Omran Abu Aboud, Bridget McLaughlin, Katie L. Anderson, Jaime F. Modiano, Kyoungmi Kim, Kuang-Yu Jen, William Senapedis, Hua Chang, Yosef Landesman, Erkan Baloglu, Roberto Pili, and Robert H. Weiss. Anti-Cancer Activity of PAK4/NAMPT Inhibitor and Programmed Cell Death Protein-1 Antibody in Kidney Cancer. Kidney360 2020 1(5):376-388. https://doi.org/10.34067/KID.0000282019

Amy E. Young, Tamer A. Mansour, Bret R. McNabb, Joseph R. Owen, Josephine F. Trott, C. Titus Brown and Alison L. Van Eenennaam. Detection and characterization of unintended alterations in the offspring of a genome edited, hornless bull. 2020. Transgenic Research, 29: 474. https://doi.org/10.1007/s11248-020-00210-1

Amy E. Young, Tamer A. Mansour, Bret R. McNabb, Joseph R. Owen, Josephine F. Trott, C. Titus Brown, Alison L. Van Eenennaam. Genomic and phenotypic analyses of six offspring of a genome-edited hornless bull. Nature Biotechnology 2020 Feb;38(2):225-232. doi: 10.1038/s41587-019-0266-0. Epub 2019 Oct 7. PMID: 31591551; PMCID: PMC7007412. 

Rosita R. Asawa, Carina Danchik, Alexey Zahkarov, Yuchi Chen, Ty Voss, Ajit Jadhav, Darren P. Wallace, Josephine F. Trott, Robert H. Weiss, Anton Simeonov, and Natalia J. Martinez. A high-throughput screening platform for Polycystic Kidney Disease (PKD) drug repurposing utilizing murine and human ADPKD cells. Scientific Reports 2020 Mar 6;10(1):4203. doi: 10.1038/s41598-020-61082-3. PMID: 32144367; PMCID: PMC7060218. 

Sadovnikova, A., Mathews, A., Garcia, S., Trott, J. F., and Hovey, R. C. Effect of a single high dose of dexamethasone on milk production, composition, and mammary gland gene expression. 2019. Journal of Dairy Science, 102(Supplement 1): 152-153.

Kim K, Trott JF, Gao G, Chapman A, Weiss RH. Plasma metabolites and lipids associate with kidney function and kidney volume in hypertensive ADPKD patients early in the disease course. BMC Nephrol. 2019 Feb 25;20(1):66. doi: 10.1186/s12882-019-1249-6. PMID: 30803434.

Trott JF, Hwang VJ, Ishimaru T, Chmiel KJ, Zhou JX, Shim K, Stewart BJ, Mahjoub MR, Jen KY, Barupal DK, Li X, Weiss RH. Arginine reprogramming in ADPKD results in arginine-dependent cystogenesis. Am J Physiol Renal Physiol. 2018 Dec 1;315(6):F1855-F1868. doi: 10.1152/ajprenal.00025.2018. Epub 2018 Oct 3. PMID: 30280600; PMCID: PMC6336982.

Berryhill GE, Trott JF, Derpinghaus AL, Hovey RC. TRIENNIAL LACTATION SYMPOSIUM/BOLFA: Dietary regulation of allometric ductal growth in the mammary glands. J Anim Sci. 2017 Dec;95(12):5664-5674. doi: 10.2527/jas2017.1901. PMID: 29293798; PMCID: PMC6292269.

Abu Aboud O, Habib SL, Trott J, Stewart B, Liang S, Chaudhari AJ, Sutcliffe J, Weiss RH. Glutamine Addiction in Kidney Cancer Suppresses Oxidative Stress and Can Be Exploited for Real-Time Imaging. Cancer Res. 2017 Dec 1;77(23):6746-6758. doi: 10.1158/0008-5472.CAN-17-0930. Epub 2017 Oct 11. PMID: 29021138; PMCID: PMC5791889.
 
Hwang VJ, Zhou X, Chen X, Trott J, Abu Aboud O, Shim K, Dionne LK, Chmiel KJ, Senapedis W, Baloglu E, Mahjoub MR, Li X, Weiss RH. Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease. Kidney Int. 2017 Oct;92(4):922-933. doi: 10.1016/j.kint.2017.03.031. Epub 2017 May 23. PMID: 28545714; PMCID: PMC5610616.

Berryhill GE, Lemay DG, Trott JF, Aimo L, Lock AL, Hovey RC. The Transcriptome of Estrogen-Independent Mammary Growth in Female Mice Reveals That Not All Mammary Glands Are Created Equally. Endocrinology. 2017 Oct 1;158(10):3126-3139. doi: 10.1210/en.2017-00395. PMID: 28938404; PMCID: PMC5659702.

Mori H, Chen JQ, Cardiff RD, Pénzváltó Z, Hubbard NE, Schuetter L, Hovey RC, Trott JF, Borowsky AD. Pathobiology of the 129:Stat1 -/- mouse model of human age-related ER-positive breast cancer with an immune infiltrate-excluded phenotype. Breast Cancer Res. 2017 Sep 2;19(1):102. doi: 10.1186/s13058-017-0892-8. PMID: 28865492; PMCID: PMC5581425.

Chen CH, Fong LWR, Yu E, Wu R, Trott JF, Weiss RH. Upregulation of MARCKS in kidney cancer and its potential as a therapeutic target. Oncogene. 2017 Jun 22;36(25):3588-3598. doi: 10.1038/onc.2016.510. Epub 2017 Feb 6. PMID: 28166200; PMCID: PMC5926797.

Berryhill GE, Miszewski SG, Trott JF, Kraft J, Lock AL, Hovey RC. Trans-Fatty Acid-Stimulated Mammary Gland Growth in Ovariectomized Mice is Fatty Acid Type and Isomer Specific. Lipids. 2017 Mar;52(3):223-233. doi: 10.1007/s11745-016-4221-2. Epub 2017 Jan 10. PMID: 28074319.

Trott JF, Kim J, Abu Aboud O, Wettersten H, Stewart B, Berryhill G, Uzal F, Hovey RC, Chen CH, Anderson K, Graef A, Sarver AL, Modiano JF, Weiss RH. Inhibiting tryptophan metabolism enhances interferon therapy in kidney cancer. Oncotarget. 2016 Oct 11;7(41):66540-66557. doi: 10.18632/oncotarget.11658. PMID: 27572319; PMCID: PMC5341819.

Josephine Trott, Katie Anderson, Jeffrey Kim, Ashley Graef, Sharon Shacham, Yosef Landesman, Aaron Sarver, Jaime Modiano and Robert H. Weiss. 2016. Abstract LB-086: Combination therapy of immune checkpoint and nuclear exporter inhibitors in a renal cell carcinoma mouse model. Cancer Res, 76(14 Supplement): Abstract nr LB-086.

VanKlompenberg MK, Manjarín R, Donovan CE, Trott JF, Hovey RC. Regulation and localization of vascular endothelial growth factor within the mammary glands during the transition from late gestation to lactation. Domest Anim Endocrinol. 2016 Jan;54:37-47. doi: 10.1016/j.domaniend.2015.09.003. Epub 2015 Sep 25. PMID: 26490114.
 
Berryhill GE, Trott JF, Hovey RC. Mammary gland development--It's not just about estrogen. J Dairy Sci. 2016 Jan;99(1):875-83. doi: 10.3168/jds.2015-10105. Epub 2015 Oct 23. Review. PubMed PMID: 26506542; PubMed Central PMCID: PMC5068565.

Rowson-Hodel AR, Manjarin R, Trott JF, Cardiff RD, Borowsky AD, Hovey RC. Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo. BMC Cancer. 2015 Jul 31;15:562. doi: 10.1186/s12885-015-1572-7. PubMed PMID: 26228788; PubMed Central PMCID: PMC4520266.

Schennink A, Trott JF, Berryhill GE, Donovan CE, Manjarin R, VanKlompenberg MK, Rowson-Hodel AR, Luis MY, Hovey RC. Alcohol intake stimulates epithelial proliferation in an authentic model of the human breast. Reprod Toxicol. 2015 Jul;54:93-100. doi: 10.1016/j.reprotox.2014.10.020. Epub 2014 Nov 1. PubMed PMID: 25450420.

Wettersten HI, Hakimi AA, Morin D, Bianchi C, Johnstone ME, Donohoe DR, Trott JF, Aboud OA, Stirdivant S, Neri B, Wolfert R, Stewart B, Perego R, Hsieh JJ, Weiss RH. Grade-Dependent Metabolic Reprogramming in Kidney Cancer Revealed by Combined Proteomics and Metabolomics Analysis. Cancer Res. 2015 Jun 15;75(12):2541-52. doi: 10.1158/0008-5472.CAN-14-1703. Epub 2015 May 7. PubMed PMID: 25952651; PubMed Central PMCID: PMC4470795.

Chen JQ, Mori H, Cardiff RD, Trott JF, Hovey RC, Hubbard NE, Engelberg JA, Tepper CG, Willis BJ, Khan IH, Ravindran RK, Chan SR, Schreiber RD, Borowsky AD. Abnormal Mammary Development in 129:STAT1-Null Mice is Stroma-Dependent. PLoS One. 2015 Jun 15;10(6):e0129895. doi: 10.1371/journal.pone.0129895. eCollection 2015. PubMed PMID: 26075897; PubMed Central PMCID: PMC4468083.

Schennink A, Trott JF, Manjarin R, Lemay DG, Freking BA, Hovey RC. Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression. J Mol Endocrinol. 2015 Feb;54(1):1-15. doi: 10.1530/JME-14-0212. Epub 2014 Oct 30. PubMed PMID: 25358647.

Trott JF, Freking BA, Hovey RC. Variation in the coding and 3' untranslated regions of the porcine prolactin receptor short form modifies protein expression and function. Anim Genet. 2014 Feb;45(1):74-86. doi: 10.1111/age.12100. Epub 2013 Dec 18. PubMed PMID: 24350599.

Schennink A, Trott JF, Freking BA, Hovey RC. A novel first exon directs hormone-sensitive transcription of the pig prolactin receptor. J Mol Endocrinol. 2013 May 9;51(1):1-13. doi: 10.1530/JME-12-0234. Print 2013. PubMed PMID: 23576686.
 
Vanklompenberg MK, Manjarin R, Trott JF, McMicking HF, Hovey RC. Late gestational hyperprolactinemia accelerates mammary epithelial cell differentiation that leads to increased milk yield. J Anim Sci. 2013 Mar;91(3):1102-11. doi: 10.2527/jas.2012-5903. Epub 2013 Jan 7. PubMed PMID: 23296835.
 
Berryhill GE, Gloviczki JM, Trott JF, Aimo L, Kraft J, Cardiff RD, Paul CT, Petrie WK, Lock AL, Hovey RC. Diet-induced metabolic change induces estrogen-independent allometric mammary growth. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16294-9. doi: 10.1073/pnas.1210527109. Epub 2012 Sep 17. PMID: 22988119; PMCID: PMC3479619.

Trott JF, Schennink A, Petrie WK, Manjarin R, VanKlompenberg MK, Hovey RC. Triennial Lactation Symposium: Prolactin: The multifaceted potentiator of mammary growth and function. J Anim Sci. 2012 May;90(5):1674-86. doi: 10.2527/jas.2011-4682. Epub 2011 Dec 28. Review. PMID: 22205663.

Trott JF, Schennink A, Hovey RC. Cloning and expression of a unique short form of the porcine prolactin receptor. J Mol Endocrinol. 2011 Jan 19;46(1):51-62. doi: 10.1677/JME-10-0101. Print 2011 Feb. PMID: 21147947.
 
Horigan KC, Trott JF, Barndollar AS, Scudder JM, Blauwiekel RM, Hovey RC. Hormone interactions confer specific proliferative and histomorphogenic responses in the porcine mammary gland. Domest Anim Endocrinol. 2009 Aug;37(2):124-38. doi: 10.1016/j.domaniend.2009.04.002. Epub 2009 May 26. PMID: 19497700.
 
Trott JF, Horigan KC, Gloviczki JM, Costa KM, Freking BA, Farmer C, Hayashi K, Spencer T, Morabito JE, Hovey RC. Tissue-specific regulation of porcine prolactin receptor expression by estrogen, progesterone, and prolactin. J Endocrinol. 2009 Jul;202(1):153-66. doi: 10.1677/JOE-08-0486. Epub 2009 Apr 28. PMID: 19401343.

Morabito JE, Trott JF, Korz DM, Fairfield HE, Buck SH, Hovey RC. A 5' distal palindrome within the mouse mammary tumor virus-long terminal repeat recruits a mammary gland-specific complex and is required for a synergistic response to progesterone plus prolactin. J Mol Endocrinol. 2008 Aug;41(2):75-90. doi: 10.1677/JME-08-0027. Epub 2008 Jun 4. PMID: 18524869.

Trott JF, Vonderhaar BK, Hovey RC. Historical perspectives of prolactin and growth hormone as mammogens, lactogens and galactagogues--agog for the future! J Mammary Gland Biol Neoplasia. 2008 Mar;13(1):3-11. doi: 10.1007/s10911-008-9064-x. Epub 2008 Jan 19. Review. PMID: 18204889.

Trott JF, Farley NR, Taatjes DJ, Hovey RC. Cloning and functional characterization of allelic variation in the porcine prolactin receptor. Domest Anim Endocrinol. 2007 Oct;33(3):313-34. Epub 2006 Jul 31. PMID: 16905289.

Trott JF, Adams TE, Wilson M, Nicholas KR. Positive and negative regulatory elements in the late lactation protein-A gene promoter from the tammar wallaby (Macropus eugenii). Biochim Biophys Acta. 2005 Apr 5;1728(1-2):65-76. PMID: 15777715.

Goldhar AS, Vonderhaar BK, Trott JF, Hovey RC. Prolactin-induced expression of vascular endothelial growth factor via Egr-1. Mol Cell Endocrinol. 2005 Mar 31;232(1-2):9-19. PMID: 15737464.

Greene EA, Trott JF. The Self-Guided Horse Facility Analysis: A Proactive Safety Education Tool for Equine Facilities. Journal of extension. 2004; 42(6).
 
Trott JF, Hovey RC, Koduri S, Vonderhaar BK. Multiple new isoforms of the human prolactin receptor gene. Adv Exp Med Biol. 2004;554:495-9. PMID: 15384631.
 
Hovey RC, Trott JF. Morphogenesis of mammary gland development. Adv Exp Med Biol. 2004;554:219-28.  PMID: 15384579.

Trott JF, Simpson KJ, Moyle RL, Hearn CM, Shaw G, Nicholas KR, Renfree MB. Maternal regulation of milk composition, milk production, and pouch young development during lactation in the tammar wallaby (Macropus eugenii ). Biol Reprod. 2003 Mar;68(3):929-36. PMID: 12604644.

Trott JF, Hovey RC, Koduri S, Vonderhaar BK. Alternative splicing to exon 11 of human prolactin receptor gene results in multiple isoforms including a secreted prolactin-binding protein. J Mol Endocrinol. 2003 Feb;30(1):31-47. PMID: 12580759.

Trott JF, Wilson MJ, Hovey RC, Shaw DC, Nicholas KR. Expression of novel lipocalin-like milk protein gene is developmentally-regulated during lactation in the tammar wallaby, Macropus eugenii. Gene. 2002 Jan 23;283(1-2):287-97. PMID: 11867236.

Hovey RC, Trott JF, Vonderhaar BK. Establishing a framework for the functional mammary gland: from endocrinology to morphology. J Mammary Gland Biol Neoplasia. 2002 Jan;7(1):17-38. Review. PMID: 12160083.

Hovey RC, Trott JF, Ginsburg E, Goldhar A, Sasaki MM, Fountain SJ, Sundararajan K, Vonderhaar BK. Transcriptional and spatiotemporal regulation of prolactin receptor mRNA and cooperativity with progesterone receptor function during ductal branch growth in the mammary gland. Dev Dyn. 2001 Oct;222(2):192-205. PMID: 11668597.

Nicholas KR, Fisher JA, Muths E, Trott J, Janssens PA, Reich C, Shaw DC. Secretion of whey acidic protein and cystatin is down regulated at mid-lactation in the red kangaroo (Macropus rufus). Comp Biochem Physiol A Mol Integr Physiol. 2001 Jul;129(4):851-8. PMID: 11440871.

Nicholas K, Simpson K, Wilson M, Trott J, Shaw D. The tammar wallaby: a model to study putative autocrine-induced changes in milk composition. J Mammary Gland Biol Neoplasia. 1997 Jul;2(3):299-310. PMID: 10882313.

Campbell PM, Trott JF, Claudianos C, Smyth KA, Russell RJ, Oakeshott JG. Biochemistry of esterases associated with organophosphate resistance in Lucilia cuprina with comparisons to putative orthologues in other Diptera. Biochem Genet. 1997 Feb;35(1-2):17-40. PMID: 9238516.